Prozzi D, Walravens K, Langedijk J P, Daus F, Kramps J A, Letesson J J
Unité de Microbiologie et Immunologie, Facultés Universitaires Notre Dame de la Paix, Namur, Belgium.
J Gen Virol. 1997 Feb;78 ( Pt 2):359-66. doi: 10.1099/0022-1317-78-2-359.
Antigenic variation among eight bovine respiratory syncytial virus (BRSV) isolates was determined using monoclonal antibodies (MAbs) specific for the attachment (G) protein. Two major (and one intermediate) subgroups were identified, as well as one strain that did not fit any pattern. The subgroups could also be differentiated on the basis of the Mr of the F protein cleavage product, F2. The nucleotide sequence of the G gene of seven of the BRSV strains was determined and compared with published G gene sequences. Subgroups A and A/B were more closely related in protein sequence than subgroups A and B or subgroups A/B and B. These results could not be correlated with those obtained by the determination of the Mr of the F2 polypeptide. Multiple sequence alignments showed a high level of amino acid identity at the inter-subgroup level (85% identity between subgroup A and subgroup B strains), similar to the intra-subgroup human (H)RSV identity, suggesting that the BRSV isolates form a continuum rather than distinct subgroups. However, unusual variability was observed within the immunodominant domain (amino acids 174-188) in contrast with the situation in HRSV strains belonging to the same subgroup.
利用针对附着（G）蛋白的单克隆抗体（MAb）测定了8株牛呼吸道合胞病毒（BRSV）分离株之间的抗原变异。鉴定出两个主要（和一个中间）亚组，以及一个不符合任何模式的毒株。这些亚组也可以根据F蛋白裂解产物F2的分子量进行区分。测定了7株BRSV毒株G基因的核苷酸序列，并与已发表的G基因序列进行比较。在蛋白质序列上，A亚组和A/B亚组比A亚组和B亚组或A/B亚组和B亚组更密切相关。这些结果与通过测定F2多肽分子量获得的结果不相关。多序列比对显示亚组间水平存在高度的氨基酸同一性（A亚组和B亚组毒株之间为85%同一性），类似于亚组内人呼吸道合胞病毒（HRSV）的同一性，这表明BRSV分离株形成一个连续体而非不同的亚组。然而，与属于同一亚组的HRSV毒株情况相反，在免疫显性区域（氨基酸174 - 188）内观察到异常的变异性。
