使用单克隆抗体对牛呼吸道合胞病毒融合蛋白（F）进行分析。

Analysis of the bovine respiratory syncytial virus fusion protein (F) using monoclonal antibodies.

作者信息

Pastey M K, Samal S K

机构信息

Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742, USA.

出版信息

Vet Microbiol. 1997 Nov;58(2-4):175-85. doi: 10.1016/s0378-1135(97)00171-5.

DOI:10.1016/s0378-1135(97)00171-5

PMID:9453129

Abstract

Seven monoclonal antibodies (MAbs) directed against bovine respiratory syncytial virus (BRSV) fusion (F) protein were produced and characterized by radioimmunoprecipitation and immunofluorescence assays. These seven MAbs together with the previously described MAbs (Beeler and Van Wyke Coelingh, 1989) to the F protein of human respiratory syncytial virus (HRSV) were used to study the antigenic variation of 12 strains of ungulate RSV. All except one MAbs specific for the HRSV-F protein reacted with ungulate RSV strains less efficiently, indicating that some epitopes are conserved, and others are not conserved on the F proteins of HRSV and BRSV strains. Three MAbs specific to the BRSV-F protein neutralized virus infectivity and reacted with all the ungulate RSV strains, suggesting that these epitopes are well conserved. Based on the reactivity of three other MAbs specific to the BRSV-F protein, ungulate RSVs could be grouped into two subgroups. The results indicated that there are antigenic variations in the F protein among ungulate RSV strains.

摘要

制备了7种针对牛呼吸道合胞病毒（BRSV）融合（F）蛋白的单克隆抗体（MAb），并通过放射免疫沉淀和免疫荧光试验对其进行了表征。这7种单克隆抗体与先前描述的针对人呼吸道合胞病毒（HRSV）F蛋白的单克隆抗体（Beeler和Van Wyke Coelingh，1989）一起用于研究12种有蹄类动物呼吸道合胞病毒（RSV）的抗原变异。除一种对HRSV-F蛋白具有特异性的单克隆抗体外，所有其他单克隆抗体与有蹄类动物RSV毒株的反应效率较低，这表明HRSV和BRSV毒株的F蛋白上有些表位是保守的，而有些则不保守。三种对BRSV-F蛋白具有特异性的单克隆抗体可中和病毒感染性，并与所有有蹄类动物RSV毒株发生反应，这表明这些表位是高度保守的。根据另外三种对BRSV-F蛋白具有特异性的单克隆抗体的反应性，有蹄类动物RSV可分为两个亚组。结果表明，有蹄类动物RSV毒株的F蛋白存在抗原变异。