Koomägi R, Stammler G, Manegold C, Mattern J, Volm M
German Cancer Research Center, Department of Oncological Diagnostics and Therapy, Heidelberg, Germany.
Cancer Lett. 1996 Dec 20;110(1-2):129-36. doi: 10.1016/s0304-3835(96)04471-0.
Twenty tumoral and peritumoral tissues from patients with lung cancer were analyzed immunohistochemically for the drug resistance-related proteins P-glycoprotein (P-170), topoisomerase II (Topo-II), glutathione S-transferase-pi (GST-pi), metallothionein (MT), heat shock protein-70 (HSP-70) and the putative regulators of resistance (ErbB1, Fos and Jun). Protein expression of Topo-II, GST-pi, MT, HSP-70, ErbB1, Fos and Jun was elevated in tumor tissue in comparison to normal tissue. The different expression of the proteins between tumoral and normal tissues was statistically significant for Topo-II (P = 0.05), MT (P = 0.03), and HSP-70 (P = 0.01), whereas ErbB1 showed a borderline significance. The expression of the proteins was frequently increased in smokers in comparison to non-smokers. In general, the increase of the proteins of smokers corresponded in tumoral and non-tumoral tissue. Different expression was only found with MT and HSP-70 which were higher in tissues of smokers.
对20例肺癌患者的肿瘤组织和瘤旁组织进行免疫组织化学分析，检测与耐药相关的蛋白质，包括P-糖蛋白（P-170）、拓扑异构酶II（Topo-II）、谷胱甘肽S-转移酶-π（GST-π）、金属硫蛋白（MT）、热休克蛋白-70（HSP-70）以及假定的耐药调节因子（ErbB1、Fos和Jun）。与正常组织相比，肿瘤组织中Topo-II、GST-π、MT、HSP-70、ErbB1、Fos和Jun的蛋白表达升高。肿瘤组织与正常组织之间这些蛋白的差异表达，对于Topo-II（P = 0.05）、MT（P = 0.03）和HSP-70（P = 0.01）具有统计学意义，而ErbB1显示出临界显著性。与不吸烟者相比，吸烟者中这些蛋白的表达经常增加。总体而言，吸烟者肿瘤组织和非肿瘤组织中这些蛋白的增加情况相对应。仅发现MT和HSP-70在吸烟者组织中的表达不同，且表达更高。
