Konishi N, Hiasa Y, Tsuzuki T, Tao M, Enomoto T, Miller G J
Second Department of Pathology, Nara Medical University, Japan.
Prostate. 1997 Jan 1;30(1):53-7. doi: 10.1002/(sici)1097-0045(19970101)30:1<53::aid-pros8>3.0.co;2-m.
Comparative studies of point mutations in K-, N-, and H-ras oncogenes were performed on prostate carcinoma from Japanese and American patients to clarify the racial difference.
We probed for mutations in 70 Japanese and 31 American specimens using polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis and immunohistochemistry for ras p21.
Within the 70 Japanese specimens, eight mutations in codon 12 of K-ras (five GGT-->GTT transversions and three CGT-->GAT transitions) and one mutation in codon 12 of the N-ras gene (a GGT-->GTT transversion) were confirmed, whereas the American samples yielded only one definable mutation, a GGT-->GAT transition, in codon 12 of K-ras.
The frequency of ras gene mutations in clinical carcinoma in Japanese men was higher than that in American men. It is suggested that there may be fundamental differences in the etiology of prostate carcinoma in Japan and the United States, perhaps based on genetics and/or environmental factors.
对日本和美国前列腺癌患者的K -、N -和H - ras癌基因中的点突变进行比较研究，以阐明种族差异。
我们使用聚合酶链反应-单链构象多态性（PCR - SSCP）分析和ras p21免疫组织化学方法，对70例日本标本和31例美国标本进行突变检测。
在70例日本标本中，确认了K - ras基因第12密码子的8个突变（5个GGT→GTT颠换和3个CGT→GAT转换）以及N - ras基因第12密码子的1个突变（GGT→GTT颠换），而美国标本仅在K - ras基因第12密码子中检测到1个可确定的突变，即GGT→GAT转换。
日本男性临床癌中ras基因突变频率高于美国男性。提示日本和美国前列腺癌的病因可能存在根本差异，可能基于遗传和/或环境因素。
