Suzuki H, Aida S, Akimoto S, Igarashi T, Yatani R, Shimazaki J
Department of Urology, School of Medicine, Chiba University.
Jpn J Cancer Res. 1994 Aug;85(8):847-52. doi: 10.1111/j.1349-7006.1994.tb02957.x.
Genetic alterations of ras oncogenes (K-, H- and N-ras) and adenomatous polyposis coli (APC) gene in tissues of prostate cancer from Japanese patients were examined using PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) analysis and direct sequencing. Tissues from 8 cases of untreated stage B prostate cancer surgically removed and from 10 cases of endocrine therapy-resistant metastatic disease obtained at autopsy were used in the present study. In four out of 18 cases (22%), ras point mutations were found, two in either codon 12 or 61 of K-ras and two in either 13 or 61 of H-ras. These point mutations were detected in one of the stage B cases (13%) and in three of the autopsy cases (30%). All these cases were poorly differentiated adenocarcinoma. In autopsy cases showing ras mutation in cancerous prostate, the same alteration was observed in metastatic tissues. No APC gene mutation was detected in any sample, although polymorphism was found in some cases. These results indicate that ras oncogene mutations are related to the progression of prostate cancer, whereas APC gene alteration is not involved in tumorigenesis and development of this cancer.
采用聚合酶链反应-单链构象多态性（PCR-SSCP）分析及直接测序法，检测日本前列腺癌患者组织中ras癌基因（K-ras、H-ras和N-ras）及腺瘤性息肉病（APC）基因的遗传改变。本研究使用了8例手术切除的未经治疗的B期前列腺癌组织及10例尸检获得的内分泌治疗抵抗性转移性疾病组织。18例中有4例（22%）发现ras点突变，其中2例为K-ras的第12或61密码子突变，2例为H-ras的第13或61密码子突变。这些点突变在1例B期病例（13%）及3例尸检病例（30%）中被检测到。所有这些病例均为低分化腺癌。在癌性前列腺组织中显示ras突变的尸检病例中，转移组织中也观察到相同改变。尽管在部分病例中发现多态性，但未在任何样本中检测到APC基因突变。这些结果表明，ras癌基因突变与前列腺癌进展相关，而APC基因改变不参与该癌症的发生发展。
