Pertile P, Baldi A, De Luca A, Bagella L, Virgilio L, Pisano M M, Giordano A
Department of Microbiology/Immunology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Cell Growth Differ. 1995 Dec;6(12):1659-64.
The product of the retinoblastoma-related human gene Rb2/p130 is highly homologous with the product of the retinoblastoma tumor suppressor gene (pRb) and Rb-related p107. this homology is shared mainly in the pocket domain, a region that seems to play a key role in the functions of these proteins. Here we report the molecular cloning and initial characterization of the cDNA encoding the murine homologue of the human Rb2/p130 gene product. The 4.8-kb cDNA encodes a protein of 1125 amino acids that shows 90% indentity to that of the human protein. The Rb2/p130 mRNA is found to be expressed in all of the adult mouse tissues examined, with the highest level being detected in kidney and skeletal muscle. For the protein characterization, we used a polyclonal antibody raised against the COOH terminus of the human Rb2/p130 protein that also recognizes the mouse protein. In developing mouse embryos, the Rb2/p130 protein is expressed as early as day 10 of gestation and reached a peak of expression around day 13 of gestation, implying a developmental regulation of the Rb2/p130 gene in murine ontogeny.
视网膜母细胞瘤相关人类基因Rb2/p130的产物与视网膜母细胞瘤肿瘤抑制基因（pRb）及Rb相关蛋白p107的产物高度同源。这种同源性主要存在于口袋结构域，该区域似乎在这些蛋白质的功能中起关键作用。在此，我们报告编码人类Rb2/p130基因产物小鼠同源物的cDNA的分子克隆及初步特征分析。这个4.8kb的cDNA编码一个由1125个氨基酸组成的蛋白质，该蛋白质与人类蛋白质的一致性为90%。发现Rb2/p130 mRNA在所检测的所有成年小鼠组织中均有表达，在肾脏和骨骼肌中检测到的水平最高。为了进行蛋白质特征分析，我们使用了一种针对人类Rb2/p130蛋白羧基末端产生的多克隆抗体，该抗体也能识别小鼠蛋白。在发育中的小鼠胚胎中，Rb2/p130蛋白早在妊娠第10天就开始表达，并在妊娠第13天左右达到表达高峰，这意味着Rb2/p130基因在小鼠个体发育过程中受到发育调控。
