Kottke-Marchant K, Veenstra A A, Marchant R E
Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195, USA.
J Biomed Mater Res. 1996 Feb;30(2):209-20. doi: 10.1002/(SICI)1097-4636(199602)30:2<209::AID-JBM11>3.0.CO;2-H.
The in vitro coagulant function of human aortic endothelial cells (HAECs) was investigated when grown on a series of polymer surfaces that ranged from hydrophobic to hydrophilic. The polymer interface materials were prepared by radiofrequency plasma polymerization from hexamethyl-disilazane, gamma-butyrolactone, and N-vinyl-2-pyrrolidone and deposited onto tissue culture Permanox. The three plasma polymers were noncytotoxic. When precoated with fibronectin (FN), HAECs on all four polymer surfaces were similar with respect to cell proliferation and coagulant function. Without FN precoating, cell proliferation and spreading increased with increasing surface hydrophilicity. Normalized production of tissue-type plasminogen activator increased with increasing hydrophilicity of the polymers during early incubation times, as did tissue plasminogen activator/plasminogen activator inhibitor-1 ratios. In comparison, normalized von Willebrand factor release decreased on the more hydrophilic surfaces. Thus, both endothelial cell growth and some coagulant/fibrinolytic functions are improved with increasing substrate hydrophilicity.
研究了人主动脉内皮细胞（HAECs）在一系列从疏水到亲水的聚合物表面生长时的体外凝血功能。聚合物界面材料通过射频等离子体聚合由六甲基二硅氮烷、γ-丁内酯和N-乙烯基-2-吡咯烷酮制备，并沉积在组织培养Permanox上。这三种等离子体聚合物无细胞毒性。当用纤连蛋白（FN）预包被时，所有四种聚合物表面上的HAECs在细胞增殖和凝血功能方面相似。没有FN预包被时，细胞增殖和铺展随着表面亲水性的增加而增加。在早期孵育期间，组织型纤溶酶原激活物的标准化产量随着聚合物亲水性的增加而增加，组织纤溶酶原激活物/纤溶酶原激活物抑制剂-1的比率也是如此。相比之下，在亲水性更强的表面上，血管性血友病因子的标准化释放减少。因此，随着底物亲水性的增加，内皮细胞生长和一些凝血/纤维蛋白溶解功能均得到改善。
