大肠杆菌中葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶和6-磷酸葡萄糖酸脱水酶基因座的遗传定位
Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli.
作者信息
Peyru G, Fraenkel D G
出版信息
J Bacteriol. 1968 Apr;95(4):1272-8. doi: 10.1128/jb.95.4.1272-1278.1968.
Abstract
The loci on the Escherichia coli genome of mutations affecting the constitutive enzymes glucose-6-phosphate dehydrogenase (zwf) and gluconate-6-phosphate dehydrogenase (gnd), and the inducible enzyme gluconate-6-phosphate dehydrase (edd), were determined by conjugation and transduction experiments, chiefly by three-factor crosses. They are in the same region of the chromosome, and their order is gnd-his-(edd, zwf)-aroD; gnd and his are cotransduceable, as are zwf and edd. The position of gnd in Salmonella typhimurium was shown to be similar to that in E. coli.
摘要
通过接合和转导实验，主要是三因子杂交，确定了大肠杆菌基因组上影响组成型酶葡萄糖-6-磷酸脱氢酶（zwf）和葡萄糖酸-6-磷酸脱氢酶（gnd）以及诱导型酶葡萄糖酸-6-磷酸脱水酶（edd）的突变位点。它们位于染色体的同一区域，顺序为gnd-his-（edd，zwf）-aroD；gnd和his可共转导，zwf和edd也可共转导。已证明鼠伤寒沙门氏菌中gnd的位置与大肠杆菌中的相似。
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