Gunji Y, Tagawa M, Matsubara H, Takenaga K, Sugaya M, Tasaki K, Maeda T, Kondo F, Nakajima K, Suzuki T, Asano T, Ochiai T, Isono K, Sakiyama S
Department of Surgery (II), School of Medicine, Chiba University, Japan.
Cancer Lett. 1996 Dec 3;109(1-2):171-6. doi: 10.1016/s0304-3835(96)04442-4.
We have examined the antitumor effect of murine colon carcinoma cells engineered to produce human interleukin-2 (IL-2) in syngeneic mice. Subcutaneous inoculation of retrovirally-transduced cells with IL-2 gene formed small tumors, but they became regressed spontaneously. Consequently, the inoculated mice showed prolonged survival. Histological examination of the tumors derived from IL-2-producers revealed predominant infiltration of macrophages around tumor necrotic masses. Thus, inoculation of IL-2-producing cells could protect the mice from subsequent subcutaneous or intraperitoneal challenges with wild-type cells, suggesting the induction of acquired immunity due to the effect of tumor vaccination.
我们研究了经基因工程改造后能产生人白细胞介素-2（IL-2）的小鼠结肠癌细胞在同基因小鼠体内的抗肿瘤作用。皮下接种携带IL-2基因的逆转录病毒转导细胞形成了小肿瘤，但这些肿瘤随后自发消退。因此，接种的小鼠生存期延长。对源自产生IL-2细胞的肿瘤进行组织学检查发现，肿瘤坏死灶周围主要是巨噬细胞浸润。因此，接种产生IL-2的细胞可保护小鼠免受随后野生型细胞皮下或腹腔注射的攻击，这表明肿瘤疫苗接种效应诱导了获得性免疫。
