Schwartzkroin P A, Altschuler R J
Brain Res. 1977 Oct 14;134(3):429-44. doi: 10.1016/0006-8993(77)90820-4.
Cells in the CA1 region of the hippocampus of kittens were studied using an in vitro slice preparation. Good quality intracellular records were obtained from over 100 cells from kittens 2 days to 4 weeks of age. Cell resistance was high in 2-day-old animals and decreased over the following 4 weeks. Both excitatory and inhibitory synaptic potentials were seen in all animals. EPSPs were only weakly effective in triggering spikes in the youngest kittens, but were greatly potentiated by repetitive stimulation at 3-10/sec; IPSPs caused a potent blockade of cell discharge in even the youngest preparations. Stimulation of the orthodromic input pathway led to a complex series of excitatory and inhibitory synaptic events which was not seen in the adult. In the 2- and 4-week-old kittens, a cell type with physiological properties different from the predominant pyramidal cell began to appear in recordings from the CA1 region. Technical difficulties inherent in in vivo recordings from neonatal animals were considerably less with the in vitro technique. Careful developmental studies may now be pursued in the slice at the single cell synaptic level.
利用体外脑片制备技术对新生小猫海马CA1区的细胞进行了研究。从2日龄至4周龄小猫的100多个细胞中获得了高质量的细胞内记录。2日龄动物的细胞电阻较高，在随后的4周内逐渐降低。在所有动物中均观察到兴奋性和抑制性突触电位。在最年幼的小猫中，兴奋性突触后电位（EPSP）在触发动作电位方面效果微弱，但在3-10次/秒的重复刺激下会大大增强；即使在最年幼的标本中，抑制性突触后电位（IPSP）也能有效阻断细胞放电。刺激顺向输入通路会导致一系列复杂的兴奋性和抑制性突触事件，这在成年动物中未见。在2周龄和4周龄的小猫中，一种生理特性不同于主要的锥体细胞的细胞类型开始出现在CA1区的记录中。与体内记录相比，体外技术在新生动物体内记录中固有的技术困难要少得多。现在可以在脑片中在单细胞突触水平上进行仔细的发育研究。
