Wu J D, Hsueh H C, Huang W T, Liu H S, Leung H W, Ho Y R, Lin M T, Lai M D
Department of Biochemistry, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.
DNA Cell Biol. 1997 Jan;16(1):17-22. doi: 10.1089/dna.1997.16.17.
Mouse liver cell lines that bear a stably integrated lactose operon repressor (lacI) gene and a Ha-ras gene linked to a lactose operator-containing SV40 early promoter were generated. When grown in medium containing more than 0.1 mM isopropyl beta-D-thiogalactoside (IPTG), the Ha-ras gene was induced up to 20-fold. Maximum induction of Ha-ras gene expression occurred after 12 h of exposure. The tumorigenicity of these cell lines in syngeneic mice was enhanced when the mice were maintained on drinking water containing 12.5 mM IPTG. Ha-ras gene expression in tumors was strongly induced in the presence of IPTG in vivo. Induction of Ha-ras gene expression in mice was consistently observed after 48 hr of exposure to drinking water containing IPTG. This system provides an approach for studying the function of oncogene in vivo as well as other genes of interest.
构建了稳定整合有乳糖操纵子阻遏物（lacI）基因和与含乳糖操纵子的SV40早期启动子相连的Ha-ras基因的小鼠肝细胞系。当在含有超过0.1 mM异丙基β-D-硫代半乳糖苷（IPTG）的培养基中培养时，Ha-ras基因被诱导高达20倍。暴露12小时后，Ha-ras基因表达达到最大诱导。当小鼠饮用含12.5 mM IPTG的饮用水时，这些细胞系在同基因小鼠中的致瘤性增强。在体内存在IPTG的情况下，肿瘤中的Ha-ras基因表达被强烈诱导。在饮用含IPTG的饮用水48小时后，在小鼠中持续观察到Ha-ras基因表达的诱导。该系统为研究体内癌基因以及其他感兴趣基因的功能提供了一种方法。
