Salomone N, Frati F, Bernini F
Department of Evolutionary Biology, University of Siena, Italy.
Exp Appl Acarol. 1996 Nov;20(11):607-15. doi: 10.1007/BF00053324.
To test the previously suggested synonymization of S. anomalus with the older S. magnus, a fragment of the mitochondrial COI gene was amplified via the polymerase chain reaction and sequenced. The sequence variation was examined in a total of 327 base pairs for four steganacarid mite populations, two of which belong to S. anomalus, one to S. magnus and one to S. hirsutus. The sequence variation suggests that S. magnus and S. anomalus are not distinct species and that the population from Fioreta (Siena) is more similar to that from Apulia than to the nearer population from the Apuan Alps. The results show the usefulness of this molecular approach as a tool for determining taxonomic status and for suggesting faunistic movements in the past millions of years.
为了验证之前提出的将异常隐头螨(S. anomalus)与较古老的大隐头螨(S. magnus)同义化的观点,通过聚合酶链反应扩增了线粒体COI基因的一个片段并进行测序。对总共4个隐头螨种群的327个碱基对中的序列变异进行了检测,其中2个种群属于异常隐头螨,1个属于大隐头螨,1个属于多毛隐头螨(S. hirsutus)。序列变异表明,大隐头螨和异常隐头螨并非不同的物种,并且来自菲奥雷塔(锡耶纳)的种群与来自普利亚的种群比与距离更近的阿普安阿尔卑斯山的种群更为相似。结果表明这种分子方法作为确定分类地位和提示过去数百万年动物区系迁移的工具是有用的。