Laser scanning cytometry in pathology of solid tumors. A review.

OBJECTIVE

To review application of laser scanning cytometry (LSC) to analyze several different parameters of human solid tumors in relation to the cell cycle.

STUDY DESIGN

Tissue sections of cytology specimens were stained for specific parameters and analyzed by LSC.

RESULTS

Examples of LSC analysis of the expression of ER, Ki-67, cyclin B1, BrdUrd and ploidy are given.

CONCLUSION

LSC provides rapid, high-precision measurement of the chosen parameters or constituents of each cell in a population of cells and correlates those measurements with the visual image of the corresponding cell.