Tsukazaki Y, Numa Y, Zhao S, Kawamoto K
Department of Neurosurgery, Kansai Medical University.
Hum Cell. 2000 Dec;13(4):221-8.
There have been few reports on investigation of the cell kinetics of brain tumors using a laser scanning cytometer (LSC). We compared DNA analysis using a flow cytometer (FCM) with that by LSC using established cell lines of brain tumors, and we demonstrated the similarity of both analytical results. The DNA-index (DI) and proliferating index (PI) of various brain tumors were determined, and the results showed that these indices reflected the malignancy of the tumors. The DI values were higher for astrocytoma grade IV than for astrocytoma grade II, frequently showing an aneuploid pattern. The determination of DNA-ploidy using LSC, together with cryopathological diagnosis, was considered to be useful for deciding the area to be resected for glioma perioperatively and to be worth applying clinically.
关于使用激光扫描细胞仪（LSC）研究脑肿瘤细胞动力学的报道很少。我们使用已建立的脑肿瘤细胞系，将流式细胞仪（FCM）的DNA分析与LSC的DNA分析进行了比较，并证明了两种分析结果的相似性。测定了各种脑肿瘤的DNA指数（DI）和增殖指数（PI），结果表明这些指数反映了肿瘤的恶性程度。IV级星形细胞瘤的DI值高于II级星形细胞瘤，常表现为非整倍体模式。使用LSC进行DNA倍体测定，结合冷冻病理诊断，被认为有助于在围手术期确定胶质瘤的切除范围，值得临床应用。
