Saarela M, Hannula J, Mättö J, Asikainen S, Alaluusua S
Research Laboratory, University of Helsinki, Finland.
Arch Oral Biol. 1996 Aug-Sep;41(8-9):821-6. doi: 10.1016/s0003-9969(96)00049-0.
The discriminative power of the arbitrarily primed polymerase chain reaction (AP-PCR) in differentiating between Streptococcus mutans and Strep. sobrinus species, serotypes and clones was investigated. Mutans streptococcal isolates (12(7)) obtained from 65 individuals (1-10 isolates per individual) were AP-PCR typed separately with two random primers, OPA-05 and OPA-13. Bacterial cell lysates were used as a template in PCR reactions, which made AP-PCR easy and fast to perform. Eighty-one isolates from 19 individuals were also ribotyped to compare the discriminative ability of ribotyping and AP-PCR techniques. AP-PCR performed with the two primers differentiated between Strep. mutans and Strep. sobrinus isolates, but neither primer detected serotype-specific amplification products. OPA-05 distinguished two main AP-PCR patterns among Strep. mutans isolates and one main pattern among Strep. sobrinus isolates, whereas OPA-13 found one main AP-PCR pattern among Strep. mutans isolates and two main patterns among Strep. sobrinus isolates. Ribotyping and AP-PCR revealed 40 and 33 different types among 81 selected isolates, respectively. Both techniques detected intra-individual heterogeneity in 16 out of 19 participants. The results indicate that AP-PCR has good discriminative ability in differentiating between mutans streptococcal clones and that the technique is suitable for epidemiological studies on mutans streptococci.
研究了任意引物聚合酶链反应（AP-PCR）在区分变形链球菌和远缘链球菌的菌种、血清型及克隆株方面的鉴别能力。从65名个体（每人1 - 10株分离株）获得的变形链球菌分离株（12(7)），分别用两种随机引物OPA - 05和OPA - 13进行AP-PCR分型。细菌细胞裂解物用作PCR反应的模板，这使得AP-PCR操作简便快捷。还对来自19名个体的81株分离株进行了核糖体分型，以比较核糖体分型和AP-PCR技术的鉴别能力。用这两种引物进行的AP-PCR能够区分变形链球菌和远缘链球菌分离株，但两种引物均未检测到血清型特异性扩增产物。OPA - 05在变形链球菌分离株中区分出两种主要的AP-PCR模式，在远缘链球菌分离株中区分出一种主要模式，而OPA - 13在变形链球菌分离株中发现一种主要的AP-PCR模式，在远缘链球菌分离株中发现两种主要模式。核糖体分型和AP-PCR分别在81株选定的分离株中揭示了40种和33种不同类型。两种技术在19名参与者中的16名中均检测到个体内异质性。结果表明，AP-PCR在区分变形链球菌克隆株方面具有良好的鉴别能力，该技术适用于变形链球菌的流行病学研究。
