Nandi S, Suryanarayana V V, Banumathi N, Sen A K
FMD Research Centre, Indian Veterinary Research Institute, Hebbal, Bangalore, India.
Comp Immunol Microbiol Infect Dis. 1997 Jan;20(1):95-9. doi: 10.1016/s0147-9571(96)00010-0.
Both polyacrylamide gel electrophoresis (PAGE) and iso-electric focusing (IEF) have been standardized using the sucrose density gradient purified 146S particles of FMD virus subtypes A10 and A22. Differences in the molecular weights of structural proteins (VP1, VP2 and VP3 of two subtypes (A10 and A22) of FMDV have been revealed in PAGE but no appreciable differences in the pI of VP1, VP2 and VP3 is found in IEF.
聚丙烯酰胺凝胶电泳（PAGE）和等电聚焦（IEF）均已通过蔗糖密度梯度纯化的口蹄疫病毒A10和A22亚型146S颗粒进行了标准化。在PAGE中已揭示了口蹄疫病毒两种亚型（A10和A22）的结构蛋白（VP1、VP2和VP3）分子量的差异，但在IEF中未发现VP1、VP2和VP3的等电点有明显差异。
