哺乳动物cDNA和原核报告基因序列会使转基因小鼠中的相邻转基因沉默。
Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.
作者信息
Clark A J, Harold G, Yull F E
机构信息
Roslin Institute, Roslin , Midlothian EH25 9PS, UK.
出版信息
Nucleic Acids Res. 1997 Mar 1;25(5):1009-14. doi: 10.1093/nar/25.5.1009.
Abstract
The ovine beta-lactoglobulin gene is expressed efficiently and at high levels in the mammary gland of transgenic mice. In contrast, when this gene is linked to a second gene construct comprising a mammalian cDNA or a CAT reporter sequence it fails to be expressed in the majority of transgenic lines generated. We suggest that mammalian cDNAs and prokaryotic reporter sequences can serve as active foci for gene silencing in the mammalian genome.
摘要
绵羊β-乳球蛋白基因在转基因小鼠的乳腺中高效且高水平表达。相比之下，当该基因与包含哺乳动物cDNA或CAT报告序列的第二个基因构建体连接时，在大多数产生的转基因系中它无法表达。我们认为哺乳动物cDNA和原核报告序列可作为哺乳动物基因组中基因沉默的活性位点。
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