二氢叶酸还原酶信使核糖核酸生成的调控
Control of dihydrofolate reductase messenger ribonucleic acid production.
作者信息
Leys E J, Kellems R E
出版信息
Mol Cell Biol. 1981 Nov;1(11):961-71. doi: 10.1128/mcb.1.11.961-971.1981.
We used methotrexate-resistant mouse cells in which dihydrofolate reductase levels are approximately 500 times normal to study the effect of growth stimulation on dihydrofolate reductase gene expression. As a result of growth stimulation, the relative rate of dihydrofolate reductase protein synthesis increased threefold, reaching a maximum between 25 and 30 h after stimulation. The relative rate of dihydrofolate reductase messenger ribonucleic acid production (i.e., the appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm) increased threefold after growth stimulation and was accompanied by a corresponding increase in the relative steady-state level of dihydrofolate reductase ribonucleic acid in the nucleus. However, the increase in the nuclear level of dihydrofolate reductase ribonucleic acid was not accompanied by a significant increase in the relative rate of transcription of the dihydrofolate reductase genes. These data indicated that the relative rate of appearance of dihydrofolate reductase messenger ribonucleic acid in the cytoplasm depends on the relative stability of the dihydrofolate reductase ribonucleic acid sequences in the nucleus and is not dependent on the relative rate of transcription of the dihydrofolate reductase genes.
我们使用了二氢叶酸还原酶水平约为正常水平500倍的甲氨蝶呤抗性小鼠细胞，来研究生长刺激对二氢叶酸还原酶基因表达的影响。作为生长刺激的结果，二氢叶酸还原酶蛋白质合成的相对速率增加了三倍，在刺激后25至30小时达到最大值。生长刺激后，二氢叶酸还原酶信使核糖核酸产生的相对速率（即细胞质中二氢叶酸还原酶信使核糖核酸的出现）增加了三倍，并伴随着细胞核中二氢叶酸还原酶核糖核酸相对稳态水平的相应增加。然而，二氢叶酸还原酶核糖核酸核水平的增加并未伴随着二氢叶酸还原酶基因转录相对速率的显著增加。这些数据表明，细胞质中二氢叶酸还原酶信使核糖核酸出现的相对速率取决于细胞核中二氢叶酸还原酶核糖核酸序列的相对稳定性，而不依赖于二氢叶酸还原酶基因的转录相对速率。
Zotero 插件