细菌RNA聚合酶主要σ因子上一种高度交叉反应性单克隆抗体的表位鉴定。
Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase.
作者信息
Breyer M J, Thompson N E, Burgess R R
机构信息
McArdle Laboratory for Cancer Research, University of Wisconsin--Madison, 53706, USA.
出版信息
J Bacteriol. 1997 Feb;179(4):1404-8. doi: 10.1128/jb.179.4.1404-1408.1997.
Abstract
A highly cross-reactive monoclonal antibody (MAb), 2G10, was found to react in a conserved region of Escherichia coli RNA polymerase sigma70. The epitope was localized to amino acids 470 to 486, which included part of conserved region 3.1. The epitope for MAb 3D3, a MAb which maps close to the 2G10 epitope, was also determined.
摘要
发现一种高度交叉反应性单克隆抗体（MAb）2G10能与大肠杆菌RNA聚合酶σ70的一个保守区域发生反应。该表位定位于氨基酸470至486，其中包括保守区域3.1的一部分。还确定了单克隆抗体3D3的表位，3D3是一种定位在接近2G10表位位置的单克隆抗体。
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