卡方凝胶对小鼠单纯疱疹病毒2型（HSV-2）皮肤感染及阿昔洛韦耐药HSV的疗效。

Efficacy of Cafon gel on cutaneous infection with herpes simplex virus (HSV)-2 and acyclovir-resistant HSV in mice.

作者信息

Yoshida Y, Yamamura J, Sato H, Koyasu M, Obara Y, Sekiguchi H, Kawana T, Shiraki K

机构信息

Department of Virology, Toyama Medical and Pharmaceutical University, Japan.

出版信息

J Dermatol Sci. 1996 Dec;13(3):237-41. doi: 10.1016/s0923-1811(97)89474-1.

DOI:10.1016/s0923-1811(97)89474-1

PMID:9023706

Abstract

Caffeine is known to inhibit replication of herpes simplex virus (HSV)-1 and the therapeutic efficacy of caffeine (Cafon) gel has been shown in a mouse model cutaneously infected with HSV-1. In this study we examined the inhibitory effect of caffeine on infection with HSV-2 and acyclovir-resistant HSV-1 strains, thymidine kinase (TK)-deficient and phosphonoacetic acid (PAA)-resistant HSV-1 in vitro and in vivo. Caffeine inhibited plaque formation of HSV-2 and acyclovir-resistant HSV-1 strains and their EC50 values ranged from 0.42 to 1.11 mg/ml. Topical treatment with Cafon gel was significantly effective in retarding the development of skin lesions caused by cutaneous infection with HSV-2 and PAA-resistant HSV-1 and in reducing the virus yield of the skin infected with TK-deficient HSV-1. The results suggested that Cafon gel would be useful for the topical treatment of cutaneous infection with HSV-2 and acyclovir-resistant HSV strains.

摘要

已知咖啡因可抑制单纯疱疹病毒1型（HSV-1）的复制，并且咖啡因（Cafon）凝胶在皮肤感染HSV-1的小鼠模型中已显示出治疗效果。在本研究中，我们在体外和体内检测了咖啡因对HSV-2感染以及对阿昔洛韦耐药的HSV-1毒株、胸苷激酶（TK）缺陷型和膦甲酸（PAA）耐药型HSV-1的抑制作用。咖啡因抑制了HSV-2和阿昔洛韦耐药型HSV-1毒株的蚀斑形成，其半数有效浓度（EC50）值在0.42至1.11毫克/毫升之间。用Cafon凝胶进行局部治疗在延缓由HSV-2和PAA耐药型HSV-1皮肤感染引起的皮肤损伤发展以及降低由TK缺陷型HSV-1感染的皮肤中的病毒产量方面具有显著效果。结果表明，Cafon凝胶可用于局部治疗HSV-2和阿昔洛韦耐药型HSV毒株的皮肤感染。