Kotchen T A, Talwalkar R T, Welch W J
Circ Res. 1977 Oct;41(4 Suppl 2):46-8. doi: 10.1161/01.res.41.4.46.
We have previously reported that the addition of plasma from normotensive human subjects to renin-renin substrate inhibits the in vitro rate of angiotensin generation. The purpose of the present study was to determine whether a renin-inhibiting factor could be identified in human plasma. Neutral lipids and phospholipids were extracted from plasma of seven normotensive control subjects. During 30-, 60-, and 180-minute incubations, neutral lipids inhibited the reaction between human renin and homologous renin substrate (P less than 0.01). Phospholipids did not affect the rate of angiotensin generation (P less than 0.05). The neutral lipid extract was further separated on silica gel chromatographic plates, and several fractions inhibited renin (P less than 0.01) Addition of each of six synthetic neutral lipids to renin-renin substrate did not affect the rate of angiotensin generation. In conclusion, several neutral lipid fractions extracted from human plasma inhibit the in vitro renin reaction.
我们之前曾报道，将血压正常的人类受试者的血浆添加到肾素-肾素底物中会抑制体外血管紧张素生成的速率。本研究的目的是确定是否能在人类血浆中鉴定出一种肾素抑制因子。从7名血压正常的对照受试者的血浆中提取中性脂质和磷脂。在30分钟、60分钟和180分钟的孵育过程中，中性脂质抑制了人类肾素与同源肾素底物之间的反应（P<0.01）。磷脂不影响血管紧张素生成的速率（P<0.05）。将中性脂质提取物在硅胶色谱板上进一步分离，几个组分抑制肾素（P<0.01）。将六种合成中性脂质中的每一种添加到肾素-肾素底物中均不影响血管紧张素生成的速率。总之，从人类血浆中提取的几个中性脂质组分抑制体外肾素反应。
