Parker C, Hutkins R W
Department of Food Science and Technology, University of Nebraska-Lincoln 68583-0919, USA.
Appl Environ Microbiol. 1997 Feb;63(2):543-6. doi: 10.1128/aem.63.2.543-546.1997.
Listeria monocytogenes transported glucose by a high-affinity phosphoenolpyruvate-dependent phosphotransferase system and a low-affinity proton motive force-mediated system. The low-affinity system (Km = 2.9 mM) was inhibited by 2-deoxyglucose and 6-deoxyglucose, whereas the high-affinity system (Km = 0.11 mM) was inhibited by 2-deoxyglucose and mannose but not 6-deoxyglucose. Cells and vesicles artificially energized with valinomycin transported glucose or 2-deoxyglucose at rates greater than those of de-energized cells, indicating that a membrane potential could drive uptake by the low-affinity system.
单核细胞增生李斯特菌通过高亲和力的磷酸烯醇丙酮酸依赖性磷酸转移酶系统和低亲和力的质子动力介导系统转运葡萄糖。低亲和力系统(Km = 2.9 mM)受到2-脱氧葡萄糖和6-脱氧葡萄糖的抑制,而高亲和力系统(Km = 0.11 mM)受到2-脱氧葡萄糖和甘露糖的抑制,但不受6-脱氧葡萄糖的抑制。用缬氨霉素人工激发的细胞和囊泡转运葡萄糖或2-脱氧葡萄糖的速率高于未激发的细胞,这表明膜电位可以驱动低亲和力系统的摄取。