大肠杆菌苯丙氨酸敏感型3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶反馈位点的突变分析
Mutational analysis of the feedback sites of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli.
作者信息
Kikuchi Y, Tsujimoto K, Kurahashi O
机构信息
Central Research Laboratory, Ajinomoto Co., Inc., Kawasaki, Japan.
出版信息
Appl Environ Microbiol. 1997 Feb;63(2):761-2. doi: 10.1128/aem.63.2.761-762.1997.
Abstract
In Escherichia coli, aroF, aroG, and aroH encode 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isozymes that are feedback inhibited by tyrosine, phenylalanine, and tryptophan, respectively. In vitro chemical mutagenesis of the cloned aroG gene was used to identify residues and regions of the polypeptide essential for phenylalanine feedback inhibition.
摘要
在大肠杆菌中，aroF、aroG和aroH编码3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶同工酶，它们分别受到酪氨酸、苯丙氨酸和色氨酸的反馈抑制。利用克隆的aroG基因进行体外化学诱变，以鉴定苯丙氨酸反馈抑制所必需的多肽残基和区域。
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