Hanai K, Satake M, Nakanishi H, Venkateswaran K
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
Appl Environ Microbiol. 1997 Feb;63(2):775-8. doi: 10.1128/aem.63.2.775-778.1997.
Six commercial kits were compared with the U.S. Food and Drug Administration (USFDA) method and the Japanese standard method for Salmonella isolation in foods. When only Salmonella serovars were tested, many of the methods performed well; however, when foods were artificially inoculated, only the USFDA method and immunomagnetic separation coupled with the xylose-lysine-brilliant green agar method (MS-XLBG) could positively detect Salmonella serovars. All seven wild-type Salmonella serovars were detected by the USFDA method, and the MS-XLBG method detected salmonellae from six samples.
将六种商用试剂盒与美国食品药品监督管理局(USFDA)方法以及日本食品中沙门氏菌分离的标准方法进行了比较。仅对沙门氏菌血清型进行检测时,许多方法表现良好;然而,当对食品进行人工接种时,只有USFDA方法以及免疫磁珠分离结合木糖赖氨酸亮绿琼脂方法(MS-XLBG)能够阳性检测出沙门氏菌血清型。USFDA方法检测出了所有七种野生型沙门氏菌血清型,MS-XLBG方法从六个样本中检测出了沙门氏菌。