Lynch Michael J B, Leon-Velarde Carlos G, McEwen Scott, Odumeru Joseph A
Laboratory Services Division, University of Guelph, 95 Stone Road West, Guelph, Ontario, Canada.
J Microbiol Methods. 2004 Aug;58(2):285-8. doi: 10.1016/j.mimet.2004.04.005.
Automated immunomagnetic separation (AIMS), using Dynabeads anti-Salmonella (Dynal, Oslo), was evaluated for its ability to detect Salmonella spp. in poultry environmental samples in comparison with standard, culture-based method (Health Canada, Health Protection Branch, MFHPB-20). AIMS was found to be more reliable in detecting Salmonella from artificially inoculated enrichment broths at low levels and exhibited a 15.5% higher sensitivity value than the culture method.
使用抗沙门氏菌磁珠(Dynal公司,奥斯陆)的自动免疫磁分离(AIMS)技术,与标准的基于培养的方法(加拿大卫生部,健康保护局,MFHPB - 20)相比,评估了其检测家禽环境样本中沙门氏菌属的能力。结果发现,AIMS在检测低水平人工接种增菌肉汤中的沙门氏菌时更可靠,其灵敏度值比培养法高15.5%。