Luk J M, Lindberg A A
Karolinska Institute, Department of Clinical Bacteriology, Huddinge Hospital, Stockholm, Sweden.
J Immunol Methods. 1991 Mar 1;137(1):1-8. doi: 10.1016/0022-1759(91)90387-u.
An immunomagnetic technique to detect and identify Salmonella serogroup C1 has been developed. Monoclonal antibodies (MAbs) specific for the O antigen 6,7 of Salmonella lipopolysaccharide (LPS) coupled to magnetic beads were used to isolate the salmonellae. Captured bacteria were easily identified by acridine orange staining and measured by enzyme immunoassays with a conjugate anti-LPS MAb as the detector probe. The whole detection process required 2-3 h and the sensitivity was 10(3)-10(4) bacteria/ml. The presence of blood (10%, v/v) or stool (1%, w/v) components did not interfere with the immunomagnetic assay performance.
已开发出一种用于检测和鉴定沙门氏菌C1血清群的免疫磁技术。将与磁珠偶联的针对沙门氏菌脂多糖（LPS）O抗原6,7的单克隆抗体（MAb）用于分离沙门氏菌。捕获的细菌通过吖啶橙染色很容易鉴定，并使用抗LPS MAb偶联物作为检测探针通过酶免疫测定法进行测量。整个检测过程需要2-3小时，灵敏度为10(3)-10(4)个细菌/毫升。血液（10%，v/v）或粪便（1%，w/v）成分的存在不会干扰免疫磁测定性能。
