Barr M C, Zou L, Long F, Hoose W A, Avery R J
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
Virology. 1997 Feb 3;228(1):84-91. doi: 10.1006/viro.1996.8358.
The nucleotide sequence and genomic organization have been determined for a highly cytopathic feline immunodeficiency virus (FIV) isolated from a Pallas' cat. The 9747-bp provirus of this virus, FIV-Oma, has typical lentivirus organization with LTRs, gag, pol, and env open reading frames (ORFs), putative vif and rev ORFs, and an ORF similar to ORF2/ORFA of domestic cat FIV isolates. Although the FIV-Oma provirus is 300 to 600 bp longer than other FIV proviruses, these additional bases are distributed throughout the genome. Phylogenetic analysis of a conserved region of the pol gene suggests that FIV-Oma is more closely related to some of the puma and lion lentiviruses than it is to domestic cat FIV isolates; however, many regions of the genome exhibit extensive nucleotide sequence divergence. None of the eight molecular proviral clones isolated from a genomic library are infectious, but we have constructed an infectious, cytopathic clone of FIV-Oma from subcloned and PCR-amplified fragments of these proviral clones. This clone will be useful for identifying the genetic determinants of FIV-Oma's biological activities.
已确定从一只兔狲分离出的一种高度致细胞病变的猫免疫缺陷病毒（FIV）的核苷酸序列和基因组结构。这种病毒FIV - Oma的9747碱基对前病毒具有典型的慢病毒结构，包含长末端重复序列（LTRs）、gag、pol和env开放阅读框（ORFs）、推定的vif和rev ORFs，以及一个与家猫FIV分离株的ORF2/ORFA相似的ORF。尽管FIV - Oma前病毒比其他FIV前病毒长300至600碱基对，但这些额外的碱基分布于整个基因组。对pol基因保守区域的系统发育分析表明，FIV - Oma与一些美洲狮和狮子慢病毒的亲缘关系比与家猫FIV分离株更近；然而，基因组的许多区域表现出广泛的核苷酸序列差异。从基因组文库中分离出的八个分子前病毒克隆均无感染性，但我们利用这些前病毒克隆的亚克隆和PCR扩增片段构建了一个具有感染性的、致细胞病变的FIV - Oma克隆。该克隆将有助于鉴定FIV - Oma生物学活性的遗传决定因素。
