热疗联合顺铂或培洛霉素对人上颌癌细胞系IMC-2的体外作用

In vitro effects of hyperthermia combined with cisplatin or peplomycin on the human maxillary carcinoma cell line IMC-2.

作者信息

Ohtsubo T, Saito H, Matsumoto H, Hayashi S, Shioura H, Kitai R, Saito T, Kano E

机构信息

Department of Otorhinolaryngology, Fukui Medical University, Japan.

出版信息

Int J Hyperthermia. 1997 Jan-Feb;13(1):59-67. doi: 10.3109/02656739709056430.

DOI:10.3109/02656739709056430

PMID:9024927

Abstract

We examined the interactive effects of hyperthermia combined with cisplatin (CDDP) (0.5 micrograms/ml) or peplomycin (PEP) (1.0 microgram/ml) on surviving fractions of human maxillary carcinoma IMC-2 cells. Either CDDP or PEP enhanced the 44 degree C thermosensitivity of thermotolerant cells after heating at 42 degrees C for 2 hours. The development of thermotolerance at 42 degrees C with either of the two drugs for 2 hours was not inhibited by CDDP, but it was partially inhibited by PEP. Moreover, for PEP throughout the entire period of 42-44 degrees C step-up heating, the 44 degree C thermosensitivity of thermotolerant cells after heating at 42 degrees C with PEP for 2 hours was enhanced similarly to that at 44 degrees C with PEP. Heating at 42 degrees C combined with either of the two drugs showed a marked interactive effect.

摘要

我们研究了热疗联合顺铂（CDDP）（0.5微克/毫升）或培普利霉素（PEP）（1.0微克/毫升）对人上颌癌IMC - 2细胞存活分数的交互作用。在42℃加热2小时后，CDDP或PEP均可增强耐热细胞在44℃的热敏感性。两种药物中的任何一种在42℃处理2小时所诱导的耐热性，不会被CDDP抑制，但会被PEP部分抑制。此外，对于PEP，在整个42 - 44℃逐步升温加热期间，经PEP在42℃加热2小时后的耐热细胞在44℃的热敏感性增强情况，与在44℃用PEP处理的情况类似。42℃加热联合两种药物中的任何一种均显示出显著的交互作用。