Ohtsubo T, Saito H, Tanaka N, Tsuzuki H, Saito T, Kano E
Department of Otolaryngology, Fukui Medical School, Japan.
Chemotherapy. 1997 Jan-Feb;43(1):43-50. doi: 10.1159/000239534.
The purpose of this study was to assess the efficacy of hyperthermia (42 or 44 degrees C) as a modifier of cis-diamminedichloroplatinum (II) (CDDP) cytotoxicity and platinum incorporation in human pharyngeal carcinoma KB cells. To maximize the interactive effect, we examined the time sequence of high (above 43 degrees C) or low (below 43 degrees C) hyperthermia and CDDP. Within the dose range of CDDP studied, there was a marked synergism between the effects of heating at 44 degrees C and subsequent CDDP exposure for 5 h. Pretreatment at 44 degrees C for 30 min or at 42 degrees C for 4 h enhanced CDDP cytotoxicity more than posttreatment at 44 degrees C for 30 min or at 42 degrees C for 4 h. However, the chemoenhancement ratio of pretreatment at 44 degrees C for 30 min was higher then that of pretreatment at 42 degrees C for 4 h, although the thermal isotoxic dose decreased the cell count to 60% under both conditions. There was a significant increase in CDDP uptake after hyperthermia at 44 degrees C. These results indicate that high hyperthermia effectively enhances subsequent CDDP cytotoxicity in human pharyngeal carcinoma KB cells.
本研究的目的是评估热疗（42或44摄氏度）作为顺二氨二氯铂（II）（CDDP）细胞毒性和铂掺入人咽癌KB细胞的修饰剂的疗效。为了使交互作用最大化，我们研究了高温（高于43摄氏度）或低温（低于43摄氏度）热疗与CDDP的时间顺序。在所研究的CDDP剂量范围内，44摄氏度加热与随后5小时的CDDP暴露之间存在显著的协同作用。44摄氏度预处理30分钟或42摄氏度预处理4小时比44摄氏度后处理30分钟或42摄氏度后处理4小时更能增强CDDP的细胞毒性。然而，44摄氏度预处理30分钟的化学增强比高于42摄氏度预处理4小时的化学增强比，尽管在两种情况下热等效毒性剂量都使细胞计数降至60%。44摄氏度热疗后CDDP摄取显著增加。这些结果表明，高温热疗可有效增强人咽癌KB细胞中随后的CDDP细胞毒性。
