Balayeva N M, Eremeeva M E, Ignatovich V F, Rudakov N V, Reschetnikova T A, Samoilenko I E, Yastrebov V K, Raoult D
Unite des Rickettsies, Faculte de Medecine, Marseille, France.
Am J Trop Med Hyg. 1996 Dec;55(6):685-92. doi: 10.4269/ajtmh.1996.55.685.
Restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction-amplified gene fragments was used to characterize 24 isolates of spotted fever group rickettsiae previously identified as Rickettsia sibirica from their serologic properties. These strains were obtained in Russia between 1946 and 1991 from humans and different species of Ixodid ticks. The RFLP analysis was performed using amplified DNA products obtained with a genus-specific primer pair derived from the R. prowazekii citrate synthase gene and two group-specific primer pairs from the R. rickettsii 190-kD and 120-kD surface protein antigen genes followed by Alu I, Pst I, and Rsa I restriction endonuclease digestions. Although some differences were detected in biological characteristics among the examined strains, only a single R. sibirica genotype was found with these molecular tools of identification.
利用聚合酶链反应扩增基因片段的限制性片段长度多态性（RFLP）分析，对24株先前根据血清学特性鉴定为西伯利亚立克次体的斑点热群立克次体分离株进行了特征分析。这些菌株于1946年至1991年间在俄罗斯从人类和不同种类的硬蜱中获得。使用从普氏立克次体柠檬酸合酶基因衍生的属特异性引物对以及从立氏立克次体190-kD和120-kD表面蛋白抗原基因衍生的两组特异性引物对获得的扩增DNA产物进行RFLP分析，随后进行Alu I、Pst I和Rsa I限制性内切酶消化。尽管在所检测的菌株之间检测到了一些生物学特性上的差异，但使用这些分子鉴定工具仅发现了单一的西伯利亚立克次体基因型。
