Dasch G A, Jackson L M
Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland 20889-5607, USA.
Ann N Y Acad Sci. 1998 Jun 29;849:11-20. doi: 10.1111/j.1749-6632.1998.tb11028.x.
The cytoplasmic 120 kDa antigen genes of 9 isolates of Rickettsia conorii (RC), 12 isolates of R. africae (RA), and 3 isolates of Israeli tick typhus rickettsiae (ISTT) were compared for restriction fragment length polymorphisms (RFLP) present in portions of the open reading frame amplified by polymerase chain reaction (PCR). Initially, DNAs from 13 species or serotypes of spotted fever group rickettsiae were used to select restriction enzymes (RE) that detected RFLP in gene fragments amplified with primer pairs 483WF/1514R and 764F/3409R. Among the R. conorii complex isolates, Dpn II gave RFLP differentiating all three serotypes. Unique RE patterns were obtained for RC with Bsr I and Hinf I, for RA with Mwo I, Pst I and Ssp I, and for ISTT with Hpa II. While RFLP typing of the 120 kDa gene permitted rapid separation of R. conorii complex isolates into three groups corresponding to the RC, RA, and ISTT rOmp serotypes, additional intragroup genetic variation was also detected in all three serotypes.
对9株康氏立克次体（RC）、12株非洲立克次体（RA）和3株以色列蜱传斑疹伤寒立克次体（ISTT）的细胞质120 kDa抗原基因进行了比较，以研究通过聚合酶链反应（PCR）扩增的开放阅读框部分中存在的限制性片段长度多态性（RFLP）。最初，使用来自13种斑点热群立克次体的DNA来选择能在使用引物对483WF/1514R和764F/3409R扩增的基因片段中检测到RFLP的限制性内切酶（RE）。在康氏立克次体复合菌株中，Dpn II产生的RFLP可区分所有三种血清型。用Bsr I和Hinf I对RC、用Mwo I、Pst I和Ssp I对RA、用Hpa II对ISTT分别获得了独特的RE模式。虽然120 kDa基因的RFLP分型能够将康氏立克次体复合菌株快速分为对应于RC、RA和ISTT rOmp血清型的三组，但在所有三种血清型中也检测到了额外的组内遗传变异。
