Walker D C, Aoyama K, Klaenhammer T R
Department of Microbiology, North Carolina State University, Raleigh 27695-7624, USA.
FEMS Microbiol Lett. 1996 May 1;138(2-3):233-7. doi: 10.1111/j.1574-6968.1996.tb08163.x.
Two strains of Lactobacillus acidophilus Group A1, the neotype ATCC 4356 and a human isolate NCFM-N2, widely used as a dietary adjunct in milk and cultured dairy products, were transformed with plasmid DNA by electroporation. The transformation characteristics exhibited by the two L acidophilus strains were found to differ markedly even though they appeared similar at the genomic level based on the DNA patterns of SmaI restriction fragments. To our knowledge, this is the first report of a consistent, reproducible transformation system of Lactobacillus acidophilus strains comprising the A1 DNA homology group.
嗜酸乳杆菌A1组的两株菌株，新类型ATCC 4356和一株人源分离株NCFM - N2，广泛用作牛奶和发酵乳制品中的膳食添加剂，通过电穿孔法用质粒DNA进行了转化。尽管基于SmaI限制性片段的DNA图谱在基因组水平上看起来相似，但发现这两株嗜酸乳杆菌菌株表现出的转化特性明显不同。据我们所知，这是关于包含A1 DNA同源组的嗜酸乳杆菌菌株一致、可重复转化系统的首次报道。
