Smirnov A F, Pavlova V A, Sleptsov M K, Steklenev E P
Genetika. 1996 Sep;32(9):1263-9.
Polymorphism of satellite DNA II and IV was studied in Bos taurus by means of Southern blotting and dot hybridization. Of primary interest is the absence of restriction fragment length polymorphism at the individual and interbreed levels. Differences in the content of satellite II in the genome are demonstrated for animals of the Kholmogorskaya and Yakutian breeds. The specific features of satellite IV organization in the bison, banteng and yak are revealed, allowing the use of the satellite as a specific genetic marker. Superposition of parental organizational types of this class of repeats is detected for the interspecies hybrids yak x cattle, banteng x cattle, and bison x cattle. At the same time, several cases of deviation from classic inheritance of such parental types in the interspecies hybrids were found.
通过Southern印迹法和点杂交技术研究了牛(Bos taurus)中卫星DNA II和IV的多态性。主要关注点是在个体和品种间水平上不存在限制性片段长度多态性。已证明霍尔莫戈尔斯卡亚(Kholmogorskaya)和雅库特(Yakutian)品种的动物在基因组中卫星II的含量存在差异。揭示了美洲野牛、爪哇野牛和牦牛中卫星IV组织的特异性特征,这使得该卫星可作为一种特异性遗传标记。在牦牛×牛、爪哇野牛×牛和美洲野牛×牛的种间杂种中检测到此类重复序列的亲本组织类型的叠加。同时,在种间杂种中发现了几例偏离此类亲本类型经典遗传模式的情况。