Semenova S K, Vasil'ev V A, Steklenev E P, Prosniak M I, Ryskov A P
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Genetika. 1999 Jan;35(1):101-4.
The (TTAGGG)4 oligonucleotide homologous to telomeric tandem repeats of human chromosomes was used for the first time as a multilocus hybridization probe for the analysis of genome variability in the two genera (Bos and Bison) of the Bovinae subfamily. DNA profiles for cattle, banteng, aurochs, and bison were obtained. Hybridization spectra were represented by the discrete individual- and species-specific bands characterized by codominant inheritance. For comparison, DNA profiles of the same samples obtained using the bacteriophage M13 DNA probe are presented. The usefulness of the microsatellite examined for the testing of pedigrees, description of intra- and interbreed variability as well as for determining relationships and the origins of the species of the Bovinae subfamily is discussed.
与人类染色体端粒串联重复序列同源的(TTAGGG)4寡核苷酸首次被用作多位点杂交探针,用于分析牛亚科两个属(牛属和美洲野牛属)的基因组变异性。获得了牛、爪哇野牛、原牛和美洲野牛的DNA图谱。杂交谱由具有共显性遗传特征的离散个体特异性和物种特异性条带表示。为作比较,还展示了使用噬菌体M13 DNA探针获得的相同样本的DNA图谱。讨论了所检测的微卫星在系谱测试、品种内和品种间变异性描述以及确定牛亚科物种的关系和起源方面的实用性。