Suppr超能文献

使用端粒标记(TTAGGG)4对牛亚科代表进行DNA指纹分析

[DNA-fingerprinting of representatives of Bovinae subfamilies using the telomere markers (TTAGGG)4].

作者信息

Semenova S K, Vasil'ev V A, Steklenev E P, Prosniak M I, Ryskov A P

机构信息

Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

出版信息

Genetika. 1999 Jan;35(1):101-4.

Abstract

The (TTAGGG)4 oligonucleotide homologous to telomeric tandem repeats of human chromosomes was used for the first time as a multilocus hybridization probe for the analysis of genome variability in the two genera (Bos and Bison) of the Bovinae subfamily. DNA profiles for cattle, banteng, aurochs, and bison were obtained. Hybridization spectra were represented by the discrete individual- and species-specific bands characterized by codominant inheritance. For comparison, DNA profiles of the same samples obtained using the bacteriophage M13 DNA probe are presented. The usefulness of the microsatellite examined for the testing of pedigrees, description of intra- and interbreed variability as well as for determining relationships and the origins of the species of the Bovinae subfamily is discussed.

摘要

与人类染色体端粒串联重复序列同源的(TTAGGG)4寡核苷酸首次被用作多位点杂交探针,用于分析牛亚科两个属(牛属和美洲野牛属)的基因组变异性。获得了牛、爪哇野牛、原牛和美洲野牛的DNA图谱。杂交谱由具有共显性遗传特征的离散个体特异性和物种特异性条带表示。为作比较,还展示了使用噬菌体M13 DNA探针获得的相同样本的DNA图谱。讨论了所检测的微卫星在系谱测试、品种内和品种间变异性描述以及确定牛亚科物种的关系和起源方面的实用性。

相似文献

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验