Kovalevskiĭ Iu V, Korenberg E I, Kutlina T V, Ustinova O A
Med Parazitol (Mosk). 1996 Oct-Dec(4):18-21.
The results of dark-field microscopy of 73 positive preparations from the unfed Ixodes persulcatus Sch. nymphs collected in the Perm Region of Russia have been analysed. The preparations were prepared by cutting the ticks placed into a physiological saline drop with needles. The numbers of Borrelia in the preparations varied from 0.4 to 173.6 per 100 microscopic fields (mean 22.9). It has been demonstrated that if all the standard conditions are fulfilled (magnification 600), drop volume 0.005 mil covering 18 x 18 mm glasses), it is practically impossible to overlook an infected nymph while examining 250 microscopic fields in such a preparation. The above-described conditions ensure valid reliable results of Borrelia calculation and their concentration measurements. The differences in the individual Borrelia infection rates in nymphs may be expressed by using the scale: low (up to 10), moderate (10.1-50), and high (above 50 Borrelia per 100 microscopic fields).
对从俄罗斯彼尔姆地区采集的未进食的全沟硬蜱若虫的73份阳性标本进行了暗视野显微镜检查结果分析。标本制备方法是用针切割置于生理盐滴中的蜱虫。每份标本中疏螺旋体的数量为每100个显微镜视野0.4至173.6个（平均22.9个）。结果表明，如果满足所有标准条件（放大倍数600、液滴体积0.005毫升、覆盖18×18毫米载玻片），在这样的一份标本中检查250个显微镜视野时，实际上不可能漏检感染的若虫。上述条件可确保疏螺旋体计数及其浓度测量结果有效可靠。若虫个体疏螺旋体感染率的差异可用以下标准表示：低（每100个显微镜视野最多10个）、中（10.1 - 50个）、高（每100个显微镜视野超过50个疏螺旋体）。
