Kovalevskiĭ Iu V, Korenberg E I, Nikitochkin I G
Med Parazitol (Mosk). 1991 May-Jun(3):18-21.
The results of dark-ground microscopy of 478 positive preparations from Ixodes persulcatus Sch. and I. ricinus L., gathered in the active natural foci of Lyme's disease in Leningrad Province, have been analysed. The preparation was prepared by cutting ticks placed into a drop of physiologic saline, with needles. It has been demonstrated that if all the standard conditions are fulfilled (magnification 600, drop volume 0.02 ml, covering glasses, 18 x 18 mm) it is practically impossible to overlook an infected tick during examination of 200-250 visual fields in such a preparation. The conditions described above ensure reliable results of Borrelia calculation and their concentration assessment. Differences in the degree of individual vector infestation may be expressed using a scale: low (up to 10), medium (10.1-50), high (50.1-250) and very high (above 250 Borrelia per 100 visual fields).
对从列宁格勒省莱姆病活跃自然疫源地采集的478份全沟硬蜱和蓖麻硬蜱阳性标本进行暗视野显微镜检查的结果进行了分析。标本制备方法是用针将蜱虫放入一滴生理盐水中，然后切割制成。结果表明，如果满足所有标准条件（放大倍数600、滴体积0.02毫升、盖玻片18×18毫米），在检查这种标本的200-250个视野时，实际上不可能漏检感染蜱。上述条件可确保可靠的疏螺旋体计数结果及其浓度评估。个体媒介感染程度的差异可用以下分级表示：低（最多10个）、中（10.1-50个）、高（50.1-250个）和非常高（每100个视野超过250个疏螺旋体）。
