Krzywicka H, Tadeusiak B, Janowska J
Zakład Zwalczania Skazeń Biologicznych, Państwowy Zakład Higieny, Warszawa.
Rocz Panstw Zakl Hig. 1996;47(3):325-32.
The sensitivity of spores B. subtilis and B. stearothermophilus to steam under pressure depended on the growth medium and duration of cultivation. B. subtilis and B. stearothermophilus produced the largest number of spores on medium with Mn and yeast extract. However the spores grown on the medium were not the most resistant. The resistant spores was growing up with the age of cultures. The highest level of resistance was obtained in the case of the medium with Ca, after 7-10 days of cultivation. The sporicidal effect of steam under pressure depended on the number of spores on the test.
枯草芽孢杆菌和嗜热脂肪芽孢杆菌的孢子对压力蒸汽的敏感性取决于生长培养基和培养时间。枯草芽孢杆菌和嗜热脂肪芽孢杆菌在含有锰和酵母提取物的培养基上产生的孢子数量最多。然而,在该培养基上生长的孢子并非最具抗性。抗性孢子随着培养时间的延长而产生。在含有钙的培养基上培养7 - 10天后,可获得最高水平的抗性。压力蒸汽的杀孢子效果取决于测试中孢子的数量。