[通过聚合酶链反应对霍乱弧菌菌株进行DNA分析]

[A DNA analysis of Vibrio cholerae strains by the polymerase chain reaction].

作者信息

Onishchenkpo G G, Glukhov A I, Grachev S V, Kiselev V I, Severin E S, Veriasov V N, Popov Iu A, Kulichenko A N, Naumov A V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1996 Jul-Aug(4):38-41.

PMID:9027173

Abstract

The method for the analysis of cholera toxin gene in V. cholerae strains was developed on the basis of polymerase chain reaction (PCR). This specific and highly sensitive method using primers affecting the site of the DNA of the operon of cholera toxin gene made it possible to identify one copy of V. cholerae genome. For the first time the content of cholera toxin gene in 4 V. cholerae (eltor) strains, obtained from the clinical material of cholera patients in Tajikistan and Dagestan, was shown with the use of PCR.

摘要

基于聚合酶链反应（PCR）开发了霍乱弧菌菌株中霍乱毒素基因的分析方法。这种使用影响霍乱毒素基因操纵子DNA位点的引物的特异性高灵敏度方法，使得鉴定霍乱弧菌基因组的一个拷贝成为可能。首次使用PCR显示了从塔吉克斯坦和达吉斯坦霍乱患者临床材料中获得的4株霍乱弧菌（埃尔托生物型）菌株中霍乱毒素基因的含量。