粪便样本中霍乱弧菌的直接检测。

Direct detection of Vibrio cholerae in stool samples.

作者信息

Varela P, Pollevick G D, Rivas M, Chinen I, Binsztein N, Frasch A C, Ugalde R A

机构信息

Instituto Nacional de Microbiología Carlos G Malbrán, Buenos Aires, Argentina.

出版信息

J Clin Microbiol. 1994 May;32(5):1246-8. doi: 10.1128/jcm.32.5.1246-1248.1994.

DOI:10.1128/jcm.32.5.1246-1248.1994

PMID:8051251

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263658/

Abstract

A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed by the two-step PCR and by an enzyme-linked immunosorbent assay using GM1 as the capture molecule showed that the former is more sensitive and gave positive results even when V. cholerae was not culturable or dead.

摘要

通过使用一种无需DNA纯化步骤的PCR程序，开发了一种直接检测粪便样本中霍乱弧菌的方法。粪便样本的稀释（1/100）可防止污染物对反应的抑制，连续进行两次PCR，第二次使用巢式引物，可达到所需的灵敏度。对通过两步PCR处理的粪便拭子样本和使用GM1作为捕获分子的酶联免疫吸附测定法所获得的结果进行比较，结果表明前者更灵敏，即使霍乱弧菌不可培养或已死亡时也能给出阳性结果。