Savilov P N
Anesteziol Reanimatol. 1996 Sep-Oct(5):64-7.
The status of the main routes of ammonium detoxication in the liver (synthesis of glutamine and urea) after its resection and hyperbaric oxygenation (HBO) was studied in 160 rats. HBO (3 sessions at 3 atm. abs.--50 min) following resection of the liver stimulated the activity of glutamine synthase and prevented the reduction of glutamate (a substrate for glutamine synthesis) level in the operated on liver. Hyperbaric oxygen activated the glutamine-dependent and non-glutamine-dependent pathways of urea synthesis in the resected liver and ensured the incorporation of glutamine amido groups in the ornithine cycle. HBO boosted the inhibitory effect of liver resection on the activity of glutamate dehydrogenase and prevented the accumulation of ammonium in the hepatocytes of resected liver. The stimulating effect of HBO on the ammonium-detoxifying function of the resected liver persisted for 11 days after the exposure.
在160只大鼠中研究了肝脏切除及高压氧治疗（HBO）后肝脏中铵解毒主要途径（谷氨酰胺和尿素合成）的状态。肝脏切除后进行HBO（3个疗程，绝对压力3个大气压，持续50分钟）刺激了谷氨酰胺合成酶的活性，并防止了手术肝脏中谷氨酸（谷氨酰胺合成的底物）水平的降低。高压氧激活了切除肝脏中尿素合成的谷氨酰胺依赖性和非谷氨酰胺依赖性途径，并确保了谷氨酰胺酰胺基团在鸟氨酸循环中的掺入。HBO增强了肝脏切除对谷氨酸脱氢酶活性的抑制作用，并防止了切除肝脏的肝细胞中铵的积累。HBO对切除肝脏铵解毒功能的刺激作用在暴露后持续11天。
