Schmidt M, Ache B W
Institut fur Biologie, Technische Universitat Berlin, Franklinstrasse 28/29, D-10587 Berlin, Germany
Cell Tissue Res. 1997 Feb;287(3):541-63. doi: 10.1007/s004410050778.
Antibodies against serotonin, dopamine, FMRF amide, substance P, and molluscan small cardioactive peptide (SCPB) were used to differentiate glomeruli and neurons in the olfactory deutocerebrum of the spiny lobster, Panulirus argus. Immunoreactivity to these antibodies identified distinct regions within individual columnar glomeruli of the olfactory lobe (OL), but not within the spherical glomeruli of the accessory lobe (AL). Glomeruli in the lateral, central, and medial layers of the AL, however, had different patterns of immunoreactivity. The immunostainings differentiated six types of local interneurons and three types of centrifugal projection neurons. Local interneurons included: (1) a "dorsal giant" neuron with serotonin- and FMRF amide-like immunoreactivity arborizing in most or all glomeruli of the OL, in the glomeruli of the medial and lateral layer of the AL and in the unstructured olfactory globular tract neuropil, (2) three large OL "core" neurons, two with serotonin-like and one with FMRF amide-like immunoreactivity innervating many OL glomeruli, (3) several hundred small, globuli-type OL "core" neurons with serotonin- and FMRF amide-like immunoreactivity, (4) thousands of small, globuli-type neurons with FMRF amide- and/or substance P-like immunoreactivity connecting the OL with the central layer of the AL, (5) thousands of small, globuli-type AL interneurons with substance P like immunoreactivity and additional arborizations in the unstructured deutocerebral tract neuropil, and (6) many small, globuli-type OL "rim" neurons with FMRF amide- and/or SCPB-like immunoreactivity. Centrifugal projection neurons included two that targeted the soma clusters and a pair of large neurons with dopamine-like immunoreactivity that originated in the lateral protocerebrum and arborized in the OL and AL glomeruli. Only few ascending projection neurons and no olfactory afferents were labeled. These results suggest that in the spiny lobster neurochemically distinct subpopulations of local interneurons constitute functionally distinct regions within individual OL glomeruli and across groups of AL glomeruli.
利用抗血清素、多巴胺、FMRF酰胺、P物质和软体动物小的心脏活性肽（SCPB）的抗体，对多刺龙虾（Panulirus argus）嗅觉中脑的肾小球和神经元进行区分。对这些抗体的免疫反应性确定了嗅觉叶（OL）单个柱状肾小球内的不同区域，但在副叶（AL）的球形肾小球内未发现。然而，AL外侧、中央和内侧层的肾小球具有不同的免疫反应模式。免疫染色区分出六种类型的局部中间神经元和三种类型的离心投射神经元。局部中间神经元包括：（1）一个“背侧巨”神经元，具有血清素和FMRF酰胺样免疫反应性，其树突分布在OL的大多数或所有肾小球、AL内侧和外侧层的肾小球以及无结构的嗅觉球状束神经纤维网中；（2）三个大的OL“核心”神经元，两个具有血清素样免疫反应性，一个具有FMRF酰胺样免疫反应性，支配许多OL肾小球；（3）数百个小的、球状型OL“核心”神经元，具有血清素和FMRF酰胺样免疫反应性；（4）数千个小的、球状型神经元，具有FMRF酰胺和/或P物质样免疫反应性，连接OL和AL的中央层；（5）数千个小的、球状型AL中间神经元，具有P物质样免疫反应性，并在无结构的中脑束神经纤维网中有额外的树突；（6）许多小的、球状型OL“边缘”神经元，具有FMRF酰胺和/或SCPB样免疫反应性。离心投射神经元包括两个靶向体细胞簇的神经元和一对具有多巴胺样免疫反应性的大神经元，它们起源于外侧原脑，并在OL和AL肾小球中形成树突。仅少数上升投射神经元被标记，且未标记嗅觉传入神经。这些结果表明，在多刺龙虾中，局部中间神经元的神经化学不同亚群在单个OL肾小球内以及跨AL肾小球组构成功能不同的区域。
