Supèr H, Pérez Sust P, Soriano E
Department of Animal and Plant Cell Biology, Faculty of Biology, University of Barcelona, Spain.
Brain Res Dev Brain Res. 1997 Jan 2;98(1):9-14. doi: 10.1016/s0165-3806(96)00154-x.
Transient Cajal-Retzius (CR) cells in layer I of the mammalian cerebral cortex are the first postmitotic neurons and they are believed to play a role in neuronal migration and lamination during cortical development. Freezing insults to the cortex of newborn mice produce cortical malformations similar to those observed in human brain disorders. Here we have used calretinin immunostaining to investigate the response of CR cells to freezing lesions of the cortical surface. Shortly after injury, CR cells disappeared from the lesioned zone. Moreover, CR cells located near the lesioned area adopted extremely fusiform shapes. At later postnatal stages (P12), CR cells were still abundant in layer I of the lesioned zone, in contrast to their almost complete loss in control animals. These results show that CR cells survive for longer developmental periods following cortical injury. Furthermore, the initial loss and later re-appearance of CR cells suggest that these neurons might migrate tangentially from the cortical areas surrounding the lesioned zone. These findings imply a role for CR cells in brain repair after cortical injury during development.
哺乳动物大脑皮层第I层的瞬时 Cajal-Retzius（CR）细胞是首批有丝分裂后的神经元，据信它们在皮层发育过程中的神经元迁移和分层中发挥作用。对新生小鼠的皮层进行冷冻损伤会产生与人类脑部疾病中观察到的类似的皮层畸形。在这里，我们使用钙视网膜蛋白免疫染色来研究CR细胞对皮层表面冷冻损伤的反应。损伤后不久，CR细胞从损伤区域消失。此外，位于损伤区域附近的CR细胞呈现出极细长的形状。在出生后的后期阶段（P12），与对照动物中几乎完全消失不同，CR细胞在损伤区域的第I层中仍然丰富。这些结果表明，CR细胞在皮层损伤后能在更长的发育时期存活。此外，CR细胞最初的消失和后来的重新出现表明，这些神经元可能从损伤区域周围的皮层区域沿切线方向迁移。这些发现暗示了CR细胞在发育过程中皮层损伤后的脑修复中的作用。
