Hostnik P, Grom J
Veterinary Faculty, Ljubijana, Slovenia.
J Wildl Dis. 1997 Jan;33(1):143-5. doi: 10.7589/0090-3558-33.1.143.
The blood-containing fluids in the thoracic cavity or blood from the heart from 177 red foxes (Vulpes vulpes) in Slovenia were evaluated for rabies antibodies by rapid fluorescent focus inhibition test (RFFIT) and an adapted indirect immunofluorescent test (IIF) in 1994. We evaluated the usefulness of anti-dog fluorescein-isothiocyanate (FITC) conjugate instead of anti-fox FITC conjugate in detection of antibodies against rabies virus in fox sera. In the RFFIT test, 92 (52%) of the fox samples were positive and 70 (40%) samples were negative for rabies antibodies; 15 (8.5%) samples were not suitable for examination in this test. In the IIF test, 98 (55%) fox samples were positive and 79 (45%) sera were negative. The IIF test was suitable for the rapid detection of antibodies against rabies virus in foxes, as often required for vaccine efficacy trials.
1994年，通过快速荧光灶抑制试验（RFFIT）和改良间接免疫荧光试验（IIF），对斯洛文尼亚177只赤狐（赤狐属）胸腔内的含血体液或心脏血液进行了狂犬病抗体评估。我们评估了用抗犬异硫氰酸荧光素（FITC）结合物代替抗狐FITC结合物检测狐血清中狂犬病病毒抗体的有效性。在RFFIT试验中，92份（52%）狐样本狂犬病抗体呈阳性，70份（40%）样本呈阴性；15份（8.5%）样本不适用于该试验检测。在IIF试验中，98份（55%）狐样本呈阳性，79份（45%）血清呈阴性。IIF试验适用于快速检测狐体内狂犬病病毒抗体，这在疫苗效力试验中经常需要。
