Iwakiri N, Uehara F, Ohba N, Tsuyama S, Murata F
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Nippon Ganka Gakkai Zasshi. 1997 Jan;101(1):83-6.
The distribution of O- and N-linked glycoconjugates in human conjunctival goblet cells was examined histochemically using biotinylated and fluorescence-labeled lectins simultaneously. Both peanut agglutinin and Erythrina cristagalli agglutinin, specific for O- and N-linked sugar chains, respectively, bound to the same goblet cell, which demonstrated that a conjunctival goblet cell produces and contains both types of glycoconjugates. Maackia amurensis lectin II, specific for sialic acid alpha 2, 3 galactose, bound to the goblet cells, while Sambueus nigra agglutinin, specific for sialic acid alpha 2, 6 galactose, did not. This observation suggested that the terminal galactosyl residue of the glycoconjugates is alpha 2, 3-sialylated in the human conjunctival goblet cells.
使用生物素化和荧光标记的凝集素同时进行组织化学检查，以研究人结膜杯状细胞中O-连接和N-连接糖缀合物的分布。分别对O-连接和N-连接糖链具有特异性的花生凝集素和刺桐凝集素，均与同一杯状细胞结合，这表明结膜杯状细胞可产生并含有这两种类型的糖缀合物。对唾液酸α2,3半乳糖具有特异性的黑接骨木凝集素II与杯状细胞结合，而对唾液酸α2,6半乳糖具有特异性的黑接骨木凝集素则不结合。该观察结果表明，糖缀合物的末端半乳糖基残基在人结膜杯状细胞中被α2,3-唾液酸化。
