Foster J D, Getchell M L, Getchell T V
Department of Physiology and Biophysics, University of Kentucky College of Medicine, Lexington 40536-00840.
Anat Rec. 1991 Apr;229(4):525-44. doi: 10.1002/ar.1092290414.
Lectin histochemistry at the light microscope level was used to determine the distribution of sugar residues in secretory cells of the olfactory mucosae of salamander, hamster, and mouse. Differences in sugar composition and distribution of glycoconjugates found in sustentacular cells and acinar cells of Bowman's glands of these three animals were characterized. Oligosaccharides in secretory products of sustentacular cells in salamander olfactory mucosa contained sialic acid, galactose (Gal), N-acetylgalactosamine (GalNAc), N-acetylglucosamine (GlcNAc), fucose, and mannose residues. Glycoconjugates of these cells lacked terminal galactosyl-beta-(1,3)N-acetylgalactose (Gal beta 1,3GalNAc) residues. The sequences Gal beta 1,3GalNAc, N-acetyllactosamine (Gal beta 1,4GlcNAc), and GalNAc were penultimate to sialic acid residues. Sustentacular cells of mouse and hamster did not appear to contain O-linked oligosaccharides but stained for mannose-containing N-linked oligosaccharides. Glycoconjugates of acinar and duct cells of Bowman's glands in the salamander, hamster, and mouse contained variable amounts of beta(1,4)GlcNAc residues, and terminal N-acetyllactosamine, Gal beta 1,3GalNAc, and GalNAc residues. In the salamander, glycoconjugates of acinar cells possessed terminal GlcNAc residues but were not sialylated, while those of hamster and mouse generally stained for sialic acid but did not possess terminal GlcNAc residues. Secretory products of a subpopulation of rodent acinar cells also contained penultimate Gal beta 1,3GalNAc residues. Staining for sialic acid, Gal, GalNAc, and GlcNAc in glycoconjugates of rodents was often limited to a sub-population of Bowman's glands. This was especially noticeable in the mouse.
利用光镜水平的凝集素组织化学方法来确定蝾螈、仓鼠和小鼠嗅黏膜分泌细胞中糖残基的分布。对这三种动物鲍曼腺支持细胞和腺泡细胞中糖缀合物的糖组成和分布差异进行了表征。蝾螈嗅黏膜支持细胞分泌产物中的寡糖含有唾液酸、半乳糖（Gal）、N-乙酰半乳糖胺（GalNAc）、N-乙酰葡糖胺（GlcNAc）、岩藻糖和甘露糖残基。这些细胞的糖缀合物缺乏末端半乳糖基-β-(1,3)N-乙酰半乳糖（Galβ1,3GalNAc）残基。Galβ1,3GalNAc、N-乙酰乳糖胺（Galβ1,4GlcNAc）和GalNAc序列是唾液酸残基的倒数第二个残基。小鼠和仓鼠的支持细胞似乎不含有O-连接寡糖，但对含甘露糖的N-连接寡糖呈阳性染色。蝾螈、仓鼠和小鼠鲍曼腺腺泡细胞和导管细胞的糖缀合物含有不同量的β(1,4)GlcNAc残基以及末端N-乙酰乳糖胺、Galβ1,3GalNAc和GalNAc残基。在蝾螈中，腺泡细胞的糖缀合物具有末端GlcNAc残基但未被唾液酸化，而仓鼠和小鼠的糖缀合物通常对唾液酸呈阳性染色但不具有末端GlcNAc残基。啮齿动物腺泡细胞亚群的分泌产物也含有倒数第二个Galβ1,3GalNAc残基。啮齿动物糖缀合物中唾液酸、Gal、GalNAc和GlcNAc的染色通常仅限于鲍曼腺的一个亚群。这在小鼠中尤为明显。
