Saliva contamination of dentin bonding agents.

This study examined the shear bond strength to dentin of two dentin bonding agents when contaminated with a measured amount of saliva at various stages in their application procedure. Eighty extracted human third molar teeth were randomly separated into four groups of 10 for each of the dentin bonding systems tested (All-Bond 2, Scotchbond Multi-Purpose). Group A specimens were not contaminated; primer/adhesive/resin were applied according to manufacturers' instructions. In Group B, samples were contaminated for 15 seconds with fresh whole human saliva, and then forcibly dried with a blast of oil-free air; this occurred after application of the primer but prior to application of adhesive. In Group C, contamination occurred after application of adhesive, prior to application of resin. In Group D, saliva was allowed to contaminate the surface as the primer was being applied, without forcible removal. Specimens were then thermocycled, mounted, and tested in shear on an Instron at 7 days. Bond strengths in MPa were obtained, and data were analyzed using a one-way ANOVA, at the P = 0.05 level of significance. Although shear bond strengths were lowered in saliva-contaminated samples, there was no statistically significant difference between group means.