Gaidano G, Pastore C, Gloghini A, Capello D, Tirelli U, Saglio G, Carbone A
Dipartimento di Scienze Mediche, Università di Torino, Italy.
Hum Pathol. 1997 Jun;28(6):748-50. doi: 10.1016/s0046-8177(97)90187-8.
Body-cavity-based lymphoma (BCBL) is a rare non-Hodgkin-'s lymphoma (NHL) type growing in liquid phase in the body cavities. Virtually all BCBL associate with Kaposi's sarcoma-associated herpesvirus/human herpesvirus type-8 infection in the absence of molecular alterations typical of other NHL categories. Microsatellite instability (MSI), a specific variant of genomic instability frequently associated with human cancers, has not been tested in BCBL thus far. In this report, we have analyzed the presence of MSI in the tumor cells of a male patient affected by AIDS-related BCBL. The genomic configuration of 12 distinct microsatellite loci was investigated by polymerase chain reaction amplification of DNA obtained from the patient's tumor cells and from autologous peripheral blood mononuclear cells. MSI was observed at 4/12 microsatellite repeats tested. Absence of the germline allele at one microsatellite locus mapping to chromosome X indicates that, in this BCBL case, MSI represents a clonal genetic lesion affecting virtually all tumor cells. These data suggest that MSI may be potentially involved in the pathogenesis of AIDS-related BCBL.
体腔淋巴瘤（BCBL）是一种罕见的非霍奇金淋巴瘤（NHL）类型，在体腔内以液相形式生长。实际上，所有BCBL都与卡波西肉瘤相关疱疹病毒/人类疱疹病毒8型感染相关，且不存在其他NHL类别的典型分子改变。微卫星不稳定性（MSI）是一种常与人类癌症相关的基因组不稳定性的特定变体，迄今为止尚未在BCBL中进行检测。在本报告中，我们分析了一名患有艾滋病相关BCBL的男性患者肿瘤细胞中MSI的存在情况。通过聚合酶链反应扩增从患者肿瘤细胞和自体外周血单个核细胞中获得的DNA，研究了12个不同微卫星位点的基因组构型。在检测的12个微卫星重复序列中有4个观察到MSI。在一个定位于X染色体的微卫星位点上未检测到种系等位基因，这表明在该BCBL病例中，MSI代表一种几乎影响所有肿瘤细胞的克隆性遗传病变。这些数据表明，MSI可能潜在地参与了艾滋病相关BCBL的发病机制。
