Foulis A K, McGill M, Farquharson M A, Hilton D A
Department of Pathology, Royal Infirmary, Glasgow, UK.
Diabetologia. 1997 Jan;40(1):53-61. doi: 10.1007/s001250050642.
Techniques were developed to look for evidence of viral infection in formalin-fixed paraffin-embedded autopsy pancreatic tissues from patients who had died of recent-onset insulin-dependent diabetes mellitus. DNA extracted from 47 pancreases in which good DNA preservation was confirmed was analysed by a polymerase chain reaction for Epstein-Barr virus and by a nested polymerase chain reaction for cytomegalovirus. Histological sections from 29 pancreases in which there was good RNA preservation were tested for the presence of enterovirus and Epstein-Barr virus using in situ hybridization techniques. Seventy-five pancreases were analysed immunohistochemically for the presence of mumps virus. None of these viruses could be detected in any of the diabetic pancreases studied. Control studies suggested that the techniques employed were as sensitive as culture done at the time of autopsy. Pancreas was available for study in 9 infants who had died of myocarditis; enterovirus was demonstrable in islets in 5 of these cases. An acute or persisting infection in the pancreas at the time of clinical onset of insulin-dependent diabetes by any of the 4 virus included in this study seems unlikely.
已开发出相关技术，用于在死于近期发病的胰岛素依赖型糖尿病患者的福尔马林固定石蜡包埋尸检胰腺组织中寻找病毒感染的证据。从47个经确认DNA保存良好的胰腺中提取的DNA，通过聚合酶链反应检测爱泼斯坦 - 巴尔病毒，并通过巢式聚合酶链反应检测巨细胞病毒。对29个RNA保存良好的胰腺的组织学切片，使用原位杂交技术检测肠道病毒和爱泼斯坦 - 巴尔病毒的存在。对75个胰腺进行免疫组织化学分析，以检测腮腺炎病毒的存在。在所研究的任何糖尿病胰腺中均未检测到这些病毒。对照研究表明，所采用的技术与尸检时进行的培养一样敏感。有9名死于心肌炎的婴儿的胰腺可供研究；其中5例在胰岛中可检测到肠道病毒。在胰岛素依赖型糖尿病临床发病时，胰腺被本研究中包含的4种病毒中的任何一种急性或持续感染似乎不太可能。
