植物中霉菌毒素赭曲霉毒素A的转化：1. 小麦和玉米细胞悬浮培养物中形成的代谢产物的分离与鉴定。
Transformation of the mycotoxin ochratoxin A in plants: 1. Isolation and identification of metabolites formed in cell suspension cultures of wheat and maize.
作者信息
Ruhland M, Engelhardt G, Schäfer W, Wallnöfer P R
机构信息
Bayerische Landesanstalt für Ernährung, Abteilung Ernährung, München, Germany.
出版信息
Nat Toxins. 1996;4(6):254-60. doi: 10.1002/(SICI)(1996)4:6<254::AID-NT2>3.0.CO;2-J.
The metabolism of the mycotoxin ochratoxin A in plant cells was investigated using cell suspension cultures of wheat and maize. A number of metabolites were detected by HPLC-chromatography with fluorescence detection. The main metabolites were ochratoxin alpha, ochratoxin A methyl ester, two isomers of hydroxyochratoxin A, and the glucosides and methyl esters of both hydroxyochratoxin A isomers. The compounds were isolated by TLC and preparative HPLC and identified by mass spectrometry and specific enzymic reactions.
利用小麦和玉米的细胞悬浮培养物，对霉菌毒素赭曲霉毒素A在植物细胞中的代谢进行了研究。通过带荧光检测的高效液相色谱法检测到了多种代谢产物。主要代谢产物为赭曲霉毒素α、赭曲霉毒素A甲酯、羟基赭曲霉毒素A的两种异构体，以及两种羟基赭曲霉毒素A异构体的糖苷和甲酯。这些化合物通过薄层层析和制备型高效液相色谱法进行分离，并通过质谱分析和特定酶促反应进行鉴定。
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