Orito E, Mizokami M, Nakano T, Wu R R, Cao K, Ohba K, Ueda R, Mukaide M, Hikiji K, Matsumoto Y, Iino S
Second Department of Medicine, Nagoya City University Medical School, Japan.
Virus Res. 1996 Dec;46(1-2):89-93. doi: 10.1016/s0168-1702(96)01379-2.
Recently, a novel hepatitis virus, GB virus C/hepatitis G virus (GBV-C/HGV), has been isolated. To elucidate the seroprevalence of chronic GBV-C/HGV infection in Japan and the phylogenetic relationship between Japanese strains and the strains previously reported, serum GBV-C/HGV RNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in 203 patients with chronic liver diseases and 200 samples of voluntary blood donors. RT-PCR was performed with primers derived from the 5'-untranslated region which were conserved between GBV-C and HGV and distant from other flaviviruses including hepatitis C virus (HCV). The nucleotide sequences were determined by the dideoxy chain termination method. The phylogenetic analysis was performed by the neighbor-joining method. In 10 (4.7%) of 203 patients with chronic liver diseases and in 1 (0.5%) of 200 blood donor samples, serum GBV-C/HGV RNA was detected. Of 10 patients, 9 patients were positive for anti-HCV and negative for HBsAg, and 1 patient was positive for HBsAg and negative for anti-HCV. The phylogenetic analysis indicated that there were three major groups which were group 1 (GBV-C), group 2 (HGV), and group 3 (a group of Japanese strains). These data indicated that (1) there was a low prevalence of GBV-C/HGV infection in Japanese patients with chronic liver diseases, (2) a high proportion of patients with GBV-C/HGV infection had chronic HCV infection however, and (3) there were at least three groups in strains of GBV-C/HGV.
最近，一种新型肝炎病毒，即GB病毒C/庚型肝炎病毒（GBV-C/HGV）已被分离出来。为了阐明日本慢性GBV-C/HGV感染的血清流行率以及日本毒株与先前报道的毒株之间的系统发育关系，我们采用逆转录聚合酶链反应（RT-PCR）对203例慢性肝病患者和200份自愿献血者样本检测血清GBV-C/HGV RNA。使用源自5'-非翻译区的引物进行RT-PCR，该区域在GBV-C和HGV之间保守，且与包括丙型肝炎病毒（HCV）在内的其他黄病毒距离较远。通过双脱氧链终止法测定核苷酸序列。采用邻接法进行系统发育分析。在203例慢性肝病患者中有10例（4.7%）以及200份献血者样本中有1例（0.5%）检测到血清GBV-C/HGV RNA。在这10例患者中，9例抗-HCV阳性且HBsAg阴性，1例HBsAg阳性且抗-HCV阴性。系统发育分析表明存在三个主要组，即第1组（GBV-C）、第2组（HGV）和第3组（一组日本毒株）。这些数据表明：（１）日本慢性肝病患者中GBV-C/HGV感染率较低；（２）然而，GBV-C/HGV感染患者中很大一部分同时患有慢性HCV感染；（３）GBV-C/HGV毒株至少存在三个组。
